Product Introduction
Dispase is a neutral protease that plays a significant role in various biological and industrial processes. It is derived from Bacillus subtilis and is known for its mild proteolytic activity. This feature makes it suitable for applications requiring a gentle touch, particularly in preserving cell integrity during dissociation. Dispase has become a valuable tool in research and pharmaceutical settings, especially for its ability to separate adherent cells and tissues without damaging them.
Production Process
The production of dispase involves the fermentation of Bacillus subtilis in a controlled environment. The fermentation process optimizes conditions such as pH and temperature to enhance enzyme production. After fermentation, the broth containing dispase is filtered, and various purification steps, including precipitation and chromatography, are employed to isolate the enzyme. The final product is then lyophilized for stability and storage. This meticulous production process ensures high enzyme purity and activity.
Effects and Functions
Dispase functions primarily by hydrolyzing peptide bonds in proteins, which assists in breaking down extracellular matrices. Its mild action helps maintain cell viability during dissociation processes. Dispase is particularly effective for isolating primary cells from tissues while preserving their functional characteristics. It facilitates experiments where intact cells are needed for further studies, such as cell behavior analysis and drug testing.
Application Scenarios
Dispase is widely used in cell culture and biotechnology labs. It is essential for isolating various cell types from tissues, especially in producing primary cultures. In tissue engineering, dispase aids in creating scaffolds by effectively separating cells without damaging them. Other applications include its use in vaccine development and protein extraction processes, where gentle proteolysis is required to maintain protein activity.
Packaging and Storage
Storage Conditions: The product should be sealed, protected from light, kept away from high temperatures, and stored in a dry, cool, and well-ventilated place.
Packaging: Bulk is available in 25 kg per fiber drum, while samples are offered in 1 kg per aluminum foil bag. Custom packaging options are available upon request.
Shipping Methods: Shipping can be done via FedEx, DHL, dedicated logistics, or sea freight consolidation.
Shelf Life: Dispase has a shelf life of two years, ensuring long-term usability when stored properly.
Monica Sun possesses extensive technical expertise and market insights in the food additives industry. She excels in designing efficient and safe additive formulations tailored to various food applications, ranging from sweeteners to functional dietary fibers. Monica has successfully assisted food manufacturers in optimizing ingredient combinations to enhance product quality and improve consumer satisfaction.
